DNA template preparation
Polyplax serrata lice (n=25) were collected from yellow-necked wood mice (Apodemus flavicollis) captured in Czech Republic (Struzna) and Germany (Baiersbronn) during 2011. Hoplopleura acanthopus lice (n=40) were obtained from common voles (Microtus arvalis) trapped in Czech Republic (Hlinsko) during 2014. All samples were stored in 96% ethanol at -20°C. Total DNA was extracted from whole louse abdomens using QIAamp DNA Micro Kit (Qiagen, Hilden, Germany) according to manufacturer protocol. DNA quality was assessed by gel electrophoresis and its’ concentration measured with Qubit High sensitivity kit.