Figure 3. Effect of IFN-γ treatment on peripheral blood
monocyte PD-L1 expression. (A) Whole blood was treated with 100 ng/mL
of LPS, 100 ng/mL of IFN-γ or both. Surface monocyte PD-L1 expression
was measured by FACS at 0 h, 6 h and 18 h. Values are mean ± S.E.M. N =
6 at each time point. * P < 0.05, Repeat Measures ANOVA with
post-hoc Holms-Sidak test. (B) Representative flow cytometry images with
the control group shown in grey and each experimental condition shown
without shading. A minimum of 20,000 events acquired for each
comparison. MFI, mean fluorescence intensity; LPS,
lipopolysaccharide; IFN-γ, interferon-gamma.