Figure 2. Inhibitory effect of secoemestrin C on the proliferation and cytokine production of Con A-stimulated liver mononuclear cells (MNCs). Liver MNCs from C57BL/6J mice were stimulated with 2 μg·mL-1Con A in the presence of secoemestrin C (SC) at the indicated concentrations. After 72 h of culture, hepatic MNC proliferation was determined by CFSE-dilution assay. Cytokine concentrations in culture supernatants were quantified by cytometric bead array kits. (A) The gating strategy for hepatic NKT and conventional T cells is displayed. (B) Bar graphs depict the percentages of TCRβ+NK1.1+ NKT cells (NKT) and TCRβ+NK1.1- conventional T cells (conT) in liver MNCs. (C, D) A representative histogram (C) and summary bar graph (D) depict the percentages of proliferated cells. (E-J) Levels of IFN-γ (E), IL-2 (F), IL-6 (G), IL-17 (H), TNF-α (I), and IL-4 (J) for the various groups are shown in the bar graphS. Bar graphs indicate means ± SEM. Data are from five separate experiments (five mice per group). **P<0.01 and***P<0.001 vs. the control (NC) group.#P<0.05 and##P<0.01 vs. the Con A-treated group.