Figure 4. Effect of secoemestrin C on serum proinflammatory cytokines in Con A-challenged mice . Serum was prepared from blood collected 8 h after Con A injection. Serum levels of IFN-γ (A), IL-2 (B), IL-6 (C), IL-17 (D), TNF-α (E), and IL-4 (F) were quantified by cytometric bead array kit. Bar graphs depict the concentrations of the indicated cytokines from the various groups. Values are presented as means ± SEM. Data shown are pooled from three separate experiments (six mice per group).**P<0.01 vs. the control (NC) group.#P<0.05 and##p < 0.01 vs. the Con A-treated group.