Figure
2. Inhibitory effect of secoemestrin C on the proliferation and cytokine
production of Con A-stimulated liver mononuclear cells (MNCs). Liver
MNCs from C57BL/6J mice were stimulated with 2 μg·mL-1Con A in the presence of secoemestrin C (SC) at the indicated
concentrations. After 72 h of culture, hepatic MNC proliferation was
determined by CFSE-dilution assay. Cytokine concentrations in culture
supernatants were quantified by cytometric bead array kits. (A) The
gating strategy for hepatic NKT and conventional T cells is displayed.
(B) Bar graphs depict the percentages of
TCRβ+NK1.1+ NKT cells (NKT) and
TCRβ+NK1.1- conventional T cells
(conT) in liver MNCs. (C, D) A representative histogram (C) and summary
bar graph (D) depict the percentages of proliferated cells. (E-J) Levels
of IFN-γ (E), IL-2 (F), IL-6 (G), IL-17 (H), TNF-α (I), and IL-4 (J) for
the various groups are shown in the bar graphS. Bar graphs indicate
means ± SEM. Data are from five separate experiments (five mice per
group). **P<0.01 and***P<0.001 vs. the control (NC) group.#P<0.05 and##P<0.01 vs. the Con A-treated group.