Sequence Data Analysis
Only uniquely mapped reads were used for further analysis.
Transcriptional level (FPKM) of all the samples were measured using
Cufflinks software
(Trapnell
et al., 2012), while differentially expressed genes (DEGs) between
different samples were estimated using Cuffdiff
(Trapnell
et al. 2012). Transcripts with a log2FC (fold change) ≥ +2 and ≤ −2, and
FDR (False Discovery Rate) value <0.05 were selected as
significant differentially expressed genes (DEGs).