PPI Network and Cluster Analysis
STRING database (Search Tool for Retrieval of Interacting Genes) was used to construct the protein-protein interaction (PPI) network of Transcription factor (TFs) using G. max as reference (Szklarczyket al. , 2014).
In order to predict candidate gene, differentially expressed genes (DEGs) screened were clustered using dChip software (Lin et al . 2004), and HeatMaps were also drawn using the same dChip software.