Quercitrin potently inhibits Ca2+mobilization
The underlying mechanism of Ca2+ mobilization in the
cytosol is crucial for platelet activation involving granule secretion
and αIIbβ3 integrin activation.(Stefanini, Roden & Bergmeier, 2009) We
examined whether quercitrin affects Ca2+ mobilization
during platelet activation. We found that compared with the findings in
vehicle-treated platelets, quercitrin significantly inhibited
intracellular Ca2+ release induced by CRP and U46619
stimulation in a concentration-dependent manner (10, 20, and 30 µM)
(Figure 3A and 3B). In addition, Ca2+ influx induced
by CRP was significantly reduced in quercitrin-treated platelets.
However, quercitrin did not affect store-operated Ca2+entry (SOCE) induced by U46619 stimulation (Figure 3B). These results
suggest that quercitrin inhibits intracellular Ca2+release and SOCE induced by CRP stimulation, but its effects are
distinct from those of U46619 stimulation.