Strain and plasmid Strain and plasmid Genotype and strain description Genotype and strain description Source or reference
Bacterial strains Bacterial strains Bacterial strains
Escherichia coli DH5α Escherichia coli DH5α Escherichia coli DH5α F- ϕ 80lacZ M15 endA recA hsdR(rk-mk-) supE thi gyrA relAΔ(lacZYA-argF)U169 Taylor et al
E. coli BL21 (DE3) E. coli BL21 (DE3) E. coli BL21 (DE3) F- ompT hsdSB (rB-mB-) gal dcm Novagen
E. coli BW25113 (DE3) E. coli BW25113 (DE3) E. coli BW25113 (DE3) F- λ- Δ(araD‐araB)567, ΔlacZ4787(::rrnB‐3), lambda-, rph‐1, Δ(rhaD_rhaB)568, hsdR514 CGSC
E. coli BL21 Star (DE3) E. coli BL21 Star (DE3) E. coli BL21 Star (DE3) F- ompT hsdSB (rB-, mB-) gal dcm rne131 (DE3) Invitrogen
E. coli yhPA001 E. coli yhPA001 E. coli yhPA001 E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA, Specr In this study
E. coli yhPA002 E. coli yhPA002 E. coli yhPA002 E. coli BL21 Star (DE3) harboring pET duet-1::pipA, Ampr In this study
E. coli yhPA003 E. coli yhPA003 E. coli yhPA003 E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA and pET duet-1::pipA, Specr/Ampr In this study
E. coli yhPA004 E. coli yhPA004 E. coli yhPA004 E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA, Specr In this study
E. coli yhPA005 E. coli yhPA005 E. coli yhPA005 E. coli BL21 Star (DE3) harboring pET duet-1::pipA::pipA, Ampr In this study
E. coli yhPA006 E. coli yhPA006 E. coli yhPA006 E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA and pET duet-1::pipA::pipA, Specr/Ampr In this study
E. coli yhPA004 [pGro7] E. coli yhPA004 [pGro7] E. coli yhPA004 [pGro7] E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA and pGro7, Specr/Cmr In this study
E. coli yhPA004 [pTf16] E. coli yhPA004 [pTf16] E. coli yhPA004 [pTf16] E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA and pTf16, Specr/Cmr In this study
E. coli yhPA004 [pKJE7] E. coli yhPA004 [pKJE7] E. coli yhPA004 [pKJE7] E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA and pKJE7, Specr/Cmr In this study
E. coli yhPA004 [LysP] E. coli yhPA004 [LysP] E. coli yhPA004 [LysP] E. coli BL21 Star (DE3) harboring pCDF duet-1::pipA::pipA and pET duet-1::lysP, Specr/Ampr In this study
Plasmids Plasmids Plasmids
pCDF duet-1 pCDF duet-1 pCDF duet-1 Specr. 2 MCS site with T7 promoter, lac operator, RBS. CloDF13 replicon. 20 to 40 copy number Novagen
pRSF duet-1 pRSF duet-1 pRSF duet-1 Kmr. 2 MCS site with T7 promoter, lac operator, RBS. RSF1030 replicon (NTP1). Over 100 copy number Novagen
pACYC duet-1 pACYC duet-1 pACYC duet-1 Cmr. 2 MCS site with T7 promoter, lac operator, RBS. P15A replicon. 10 to 12 copy number Novagen
pET duet-1 pET duet-1 pET duet-1 Ampr. 2 MCS site with T7 promoter, lac operator, RBS. colE1(PBR322) replicon. 40 copy number Novagen
pGro7 pGro7 pGro7 Cmr. araB promoter::groES::groEL induced by L-arabinose. pACYC184 replicon Takara
pTf16 pTf16 pTf16 Cmr. araB promoter::tig induced by L-arabinose. pACYC184 replicon Takara
pKJE7 pKJE7 pKJE7 Cmr. araB promoter::dnaK::dnaJ::dnaE induced by L-arabinose. pACYC184 replicon Takara
pCDF duet-1::pipA pCDF duet-1::pipA pCDF duet-1::pipA pipA gene of Streptomyces pristinaespiralis ATCC25486 inserted into pCDF duet‐1 In this study
pCDF duet-1::pipA::pipA pCDF duet-1::pipA::pipA pCDF duet-1::pipA::pipA pipA gene of Streptomyces pristinaespiralis ATCC25486 inserted into pCDF duet‐1::pipA In this study
pET duet-1::pipA pET duet-1::pipA pET duet-1::pipA pipA gene of Streptomyces pristinaespiralis ATCC25486 inserted into pET duet‐1 In this study
pET duet-1::pipA::pipA pET duet-1::pipA::pipA pET duet-1::pipA::pipA pipA gene of Streptomyces pristinaespiralis ATCC25486 inserted into pET duet‐1::pipA In this study
pET duet-1::lysP pET duet-1::lysP lysP gene of E. coli K12 inserted into pET duet-1 lysP gene of E. coli K12 inserted into pET duet-1 In this study