The tetrazolium salt assay
The cytotoxic effect of ruxolitinib and FP over 24 h was evaluated using
a tetrazolium salt assay, which involved
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT; Wako
Pure Chemical). This was dissolved in BEBM at a concentration of 0.5
mg/mL and incubated with the cells for 2 h. After the supernatant had
been removed, DMSO was added and the cells were placed on a shaking
platform for 30 min. Absorbance at 570 nm was then measured for
duplicate 100 μL samples using an iMark Microplate Reader.