3.2 De novo genome assembly using Nanopore long reads
After filtering raw reads, the remaining clean reads were assembled into contigs and scaffolds using Illumina data and Nanopore data. A total of 213 scaffolds were generated with N50 size of 7.15 Mb (Table S5). We identified 1,375 complete BUSCOs, including 1,104 duplicated BUSCOs, 71 fragmented BUSCOs, and 1,160 single-copy in the assembled Chinese walnut genome (Table S6). There were 40 genes recognized as missing BUSCOs in the assembled genome (Table S6). Overall, we obtained ~548 Mb of Chinese walnut genome based on long reads, which is about 94. 8 % of the survey genome (548 Mb) (Table 1).