3.3 Chromosome-level assembly of Hi-C data
A total of 0.54 Gb assembled scaffold sequence was correctly divided
into sixteen groups corresponding to the sixteen Chinese walnut
chromosomes (Figure 2c; Figure S1). A total of 397 contigs and 189
scaffolds were generated by Hi-C sequencing data; the N50 size of
contigs was 6.49 Mb and the N50 size of scaffolds was 36.1 Mb,
respectively (Table 1). Hi-C sequence (543
Mb) was mapped and anchored ( 99
% ;543 Mb/548 Mb) to the assembled 16 chromosomes of the Chinese walnut
genome (Table 1).Chromosome numbering for J. cathayensis was
based on homology to the numbering of J. regia chromosomes (Zhang
et al., 2020) (Table S7). The lengths of the 16 assembled chromosomes of
Chinese walnut ranged from 19,675,958 bp to 55,052,647 bp with mean
length is 33,963,507 bp, while chromosomes of Persian walnut ranged from
20,184,194 bp to 518,39,233 bp with mean length is 33,799,624 bp (Table
S7).