All of the mutations that occurred were noted along with their
respective chromosome number, zygosity, and gene name. The gender of
each patient was also taken into consideration in order to determine
whether uveal melanoma is a sex-linked disorder. However, the variation
type was not significant, as that had already been investigated by other
studies.
Pareto analysis was performed to visualize the frequency of the specific
genetic mutations, as well as the chromosomes on which they were
present. A Pareto chart is a bar chart in which the bars are ordered
from highest frequency of occurrence to lowest frequency of occurrence
[7]. This statistical technique was helpful in understanding the
results of the study. An individual pareto chart regarding chromosome
involvement was generated for both the primary and metastases samples
each [Figure 2 & 3]. These charts provided information on the
number and frequency of genetic mutations per chromosome in decreasing
order. Pareto analysis of mutated genes was only run for the primary
samples [Figure 4] because the results revealed that there were no
recurring genetic mutations among the patients whose cells had undergone
metastasis.