Methodology
For the analysis, the SNV (Single-Nucleotide Variant) files were examined from the data package. The data files contained information which provided the locus of each mutation, the zygosity, variation type, and the gene on which it occurred. The ‘Match’ column was not present within the data and was created with a formula as a detection method for any coding mutations. The formula was an IF function which compared each cell from the reference sequence with the corresponding cell from the allele 2 sequence. Whenever there was a mismatch, the function would automatically flag each mutation with an ‘X’ symbol in the corresponding cell within the ‘Match’ column. After the IF function was imported into the ‘Match’ column, it was applied to the entire genome to search for any mutations. Once the comparison was complete, the ‘Match’ column was filtered out to only show the base pair mismatches (occurred only when an ‘X’ was present in the ‘Match’ column) [Figure 1]. These procedures were followed for both the primary (PUM) and metastases (MUM) files.