All of the mutations that occurred were noted along with their respective chromosome number, zygosity, and gene name. The gender of each patient was also taken into consideration in order to determine whether uveal melanoma is a sex-linked disorder. However, the variation type was not significant, as that had already been investigated by other studies.
Pareto analysis was performed to visualize the frequency of the specific genetic mutations, as well as the chromosomes on which they were present. A Pareto chart is a bar chart in which the bars are ordered from highest frequency of occurrence to lowest frequency of occurrence [7]. This statistical technique was helpful in understanding the results of the study. An individual pareto chart regarding chromosome involvement was generated for both the primary and metastases samples each [Figure 2 & 3]. These charts provided information on the number and frequency of genetic mutations per chromosome in decreasing order. Pareto analysis of mutated genes was only run for the primary samples [Figure 4] because the results revealed that there were no recurring genetic mutations among the patients whose cells had undergone metastasis.