Figure 4: Construction and characterization of P. taiwanensis VLB120 (pSEVA_6HA_2). (A) graphical representation of expression units in the plasmid pSEVA_6HA_2. (B) Time courses for the 6HA concentration and the whole-cell activity for 6HA formation in resting cell bioconversions as described in the legend of Figure 3. (C) SDS-PAGE analysis showing bands for Cyp (47.4 kDa), CDH (26.5 kDa), CHMO (58.8 kDa), and Lact (32.2 kDa) after different times of induction and compared with cells containing an empty vector. (D) Relative growth rate of P. taiwanensis VLB120 in the presence of varying amounts of ε-CL (green) or 6HA (orange) (a growth rate of 0.47 ± 0.01 h-1 represents 100 %) (E) Conversion of 5 mM cyclohexane by P. taiwanensis VLB120 containing pSEVA_CL_2 or pSEVA_6HA_2. Resting cell bioconversions were performed in 40 mL KPi-g buffer containing 1.05 gCDW L-1of cells in closed 250 mL screw-capped and baffled shake flasks. Graphs represent average values and standard deviations of two independent biological replicates.