Figure 2: Inhibition studies with P. taiwanensis VLB120
(pSEVA_CHMO). The influence of cyclohexanol (A) or the product ε-CL (B)
on specific CHMO activity was investigated in resting cell
bioconversions. Cells were cultivated in M9* medium with 0.5 % (w/v)
glucose, induced by IPTG for 6h, harvested, and resuspended in KPi-g
buffer (100 mM potassium phosphate buffer supplemented with 1 % (w/v)
glucose) to a biomass concentration of 0.25
gCDW L-1 in 1 mL liquid volume (A) or
0.5 gCDW L-1 in 10 mL liquid volume
(B). Reactions were started by adding 3 mM cyclohexanone. Graphs
represent average values and standard deviations of two independent
biological replicates.