2.1 Bacterial strains, plasmids, media, and chemicals
Microbial strains and plasmids used in this work are listed in Table S1.
Cells were grown in lysogeny broth (LB) medium[19] or M9* medium [20] with
a pH of 7.2 supplemented with 0.5 % (w/v) glucose as sole carbon and
energy source. Kanamycin (50 µg mL−1) was applied for
selection when necessary. Unless stated otherwise, all chemicals were
purchased from Sigma-Aldrich (Steinheim, Germany) or Carl Roth
(Karlsruhe, Germany) in the highest purity available and used without
further purification. 6HA was acquired from abcr (Karlsruhe, Germany).
Molecular biology methods and strain constructions are explained in
detail in the Supplementary Information (Sections 1 and 2)