Background and Purpose The active ingredients analysis of herbal medicines is crucial for their quality control and efficacy/safety evaluation. Here, we propose a new strategy for effectively and rapidly analyzing the active ingredients of herbal medicines based on the public bioinformatics platforms. In the study, the treatment of osteoarthritis (OA) by Herba Lysimachiae (HL) was used as an example to practice this strategy. Experimental Approach Applying the public databases (PubChem BioAssay and STRING) to establish the links between herbal compounds and the biolabels (integrin alpha 2b/beta 3) of HL treating OA, from which herbal compounds that may regulate both biolabels were screened. OA model was used to confirm the analysis results. Key Results Five compounds (myricetin, fisetin, esculetin, 7-hydroxycoumarin-4-acetic acid, and caffeic acid) from HL synergistically regulated both biolabels through eleven targets, which may be the active ingredients of HL treating OA. Conclusion and Implications The experiments in the OA model verified the analysis results. With the help of the analysis strategy, the present study effectively and rapidly analyzes the active ingredients of HL treating OA. The example in this research also verifies the feasibility of this strategy, which is of reference significance to the related studies of other herbal medicines.