2.5 One-step growth curve experiment
To investigate the growth characteristics of YNG strain in vitro,
monolayer Vero and PK15 cells seeded in 24-well plates were incubated
with different strains (YNG and YNP, an isolation from pig farms where
was close to this PRV-outbreaking goat farm) at multiplicity of
infection (MOI) of 0.01 for 1 h. Both the cell debris and supernatant
were collected after repeated freezing and thawing cycles, the viral
copies and titers were determined via real-time PCR after the DNA
extraction and TCID50 assay, respectively.