2.6 Sequence alignment and phylogenetic analysis
To analyze the genetic characteristics of PRV strain isolated from goat
in the present study, the major viral immune (gB , gC , andgD ) and virulence genes (gE and TK ) of YNG strain
were amplified by PCR with specific primers (Tab. 1 ). Positive
PCR products were purified, sequenced by Tsingke company (Changsha,
China), these sequences were submitted to the Genbank database. These
newly sequences, together with their corresponding reference sequences
downloaded from GenBank database, were aligned using DNAStar version
7.10 (Lasergene) software for comparison. Phylogenetic trees were also
re-constructed using the neighbor-joining (NJ) method in MEGA 7.0
software with 1000 bootstrap replicates based on the gC andgE gene, respectively.