2.8 Experimental infection of mice
Twenty-four 5-week-old female Kunming mice purchased from Hunan SJA
laboratory animal CO., LTD were randomly divided into four groups, six
mice in each group were challenged with 104TCID50 of various PRV strains (YNG, YNP, and another
classical PRV strain genetically close to Ea strain, HNLY) via hind
footpad injection, respectively, while the mice in the control group
were inoculated with equal volume of DMEM. These mice in distinct groups
were maintained in the same environment, and their health conditions
were monitored three times per day. The survival curve of mice in each
group was produced by using the GraphPad Prism 8.0 software (GraphPad
software, La Jolla, CA, USA).