3.4 Comparison between pseudotyped EBOV-based and authentic
EBOV-based neutralization assay
Equine immunoglobulin fragments against EBOV, which were produced
previously (Zheng et al., 2016), were
used as a positive control for the optimization of the pseudotyped
EBOV-based neutralization assay. After optimization, the pseudotyped
EBOV-based neutralization assay was used to measure the neutralization
activity of the immunoglobulin fragments, and the neutralization assay
based on authentic EBOV was also performed. As expected similar results
were acquired between the two different assays (Fig. 3a). Both
NT50 values were higher than 1:20,000 (1:20,480 for
pseudotyped EBOV; 1:21,333 for live EBOV), suggesting that the
pseudotyped EBOV could be used in neutralization assay with similar
infectivity compared to authentic and live EBOV, but with a reduced
health risk.