2.3 Sample collection and 16S ribosomal RNA (rRNA) high-throughput sequencing
Cecum and jejunum contents were quickly collected in 5 ml cryotubes following slaughter in each case. Intestinal contents samples were collected in five tubes and quickly placed in liquid nitrogen to be stored for subsequent intestinal 16S rRNA microbial detection and analysis. Intestinal microbial DNA extraction, detection, PCR amplification, and product purification from different intestinal samples was carried out before 16S rRNA gene sequencing was performed at Nuohe Biotechnology Co., Ltd.