3.1 16S rRNA sequencing results at different dietary protein
levels
High-throughput 16S rRNA sequencing was performed to assess gut microbes
in Bamei pigs fed with different levels of dietary protein. A total of
1,667,502 valid sequences were detected in 18 samples from three groups
which had an average length of 413 base pairs (bp). Amongst the eight
Bamei pig jejunums with different dietary protein levels, nine samples
were sequenced to obtain 847,451 pairs of reads. Once double-end reads
had been spliced and filtered, 760,071 optimized sequence numbers (Clean
Tags) were generated. These Clean Tags were filtered into chimeras to
obtain effective sequence numbers (Effective Tags); data show that each
sample generates at least 60,806 Effective Tags, 64,386 on average.
Similarly, 820,051 pairs of reads were obtained from nine cecal contents
samples. This resulted in 767,631 Clean Tags generated after double-end
reads were spliced and subjected to filtration. These Clean Tags were
then also filtered into chimeras to obtain effective sequence numbers
(Effective Tags). Data show that each sample generates at least 54,142
Effective Tags, 61,382 on average.
Table 4 Jejunum sample sequence statistics