3.1 16S rRNA sequencing results at different dietary protein levels
High-throughput 16S rRNA sequencing was performed to assess gut microbes in Bamei pigs fed with different levels of dietary protein. A total of 1,667,502 valid sequences were detected in 18 samples from three groups which had an average length of 413 base pairs (bp). Amongst the eight Bamei pig jejunums with different dietary protein levels, nine samples were sequenced to obtain 847,451 pairs of reads. Once double-end reads had been spliced and filtered, 760,071 optimized sequence numbers (Clean Tags) were generated. These Clean Tags were filtered into chimeras to obtain effective sequence numbers (Effective Tags); data show that each sample generates at least 60,806 Effective Tags, 64,386 on average. Similarly, 820,051 pairs of reads were obtained from nine cecal contents samples. This resulted in 767,631 Clean Tags generated after double-end reads were spliced and subjected to filtration. These Clean Tags were then also filtered into chimeras to obtain effective sequence numbers (Effective Tags). Data show that each sample generates at least 54,142 Effective Tags, 61,382 on average.
Table 4 Jejunum sample sequence statistics