Main Figure Legends
Figure 1: scRNA-seq of myeloid cells. (A) UMAP dimensionality
reduction and clustering of myeloid cells based on transcriptional
profiles segregated by AD subjects with dermotype A (AD_A; n = 4) and B
(AD_B; n = 5) and healthy controls with dermotype A (Healthy_A; n =
4). Cell subsets were delineated based on KNN (k-nearest neighbours)
clustering and identified using DISCO CELLiD and differentially
expressed genes (DEGs). Clusters not of myeloid origin and low cell
counts were removed before downsampling for normalisation. (B) Heatmap
showing the relative expression of DEGs from each cluster represented by
logFC values. (C) Proportion of cells represented by each cluster. (D)
Heatmap of relative expression of top DEGs from cluster 4 between AD_A
and AD_B (min.pct = 0.25 and logFC.threshold = 0.25). Expression is
shown in terms of Z-scores.
Figure 2: Differences in circulatory inflammation in PBMCs and
plasma. (A-F) Cytoflex analysis of (A) CCL3+ CCL4+ and (B) TNF-a+
IL-1b+ proportions secreted by classical monocytes from PBMCs across
dermotype A healthy (Healthy_A; n = 8) and AD subjects (AD_A; n = 5)
and dermotype B AD subjects (AD_B; n = 6). Proportions of (C) Th2, (D)
Th2a, (E) NK cells and (F) ILC3 in PBMCs measured by flow cytometry.
(G-J) Scatter plots depicting inflammatory protein biomarkers
statistically significant across Healthy_A (n = 13), AD_A (n = 16) and
AD_B (n = 10) subjects: (G) TRANCE, (H) TRAIL, (I) MCP-4 (J) IL-18.
Expression reported as normalised protein expression (NPX) values. For
all graphs, topmost line depicts statistical significance by
Kruskal-Wallis across all 3 groups while brackets depict P-values of
paired Mann-Whitney U (* P< 0.05, **P < 0.01 and **P
< 0.001).