lipases
To select the lipase suitable for hydrolysis of 3-MCPD esters, the recovery of 3-MCPD esters was also analyzed using lipase from Candida rugosa , lipase from Candida sp. and lipase from Thermophila sparsiformis at 150 U/mL. All lipases were prepared at 150 U/mL in 30% NaBr buffer, and a 0.5 h lipolysis was performed at the optimal pH and temperature proposed by the manufacturer in triplicate. As shown in Table 1, 3-MCPD esters could not be enzymatically decomposed by lipase from Thermophila sparsiformis. Among the lipases tested, lipase from Candida sp. gave the highest recovery rate for 3-MCPD. These results suggested that lipase from Candida sp. had higher substrate specificity for hydrolysis of 3-MCPD esters than other lipases tested.