lipases
To select the lipase suitable for hydrolysis of
3-MCPD
esters, the recovery of 3-MCPD
esters was also analyzed using
lipase from Candida rugosa , lipase
from Candida sp. and
lipase from Thermophila sparsiformis
at 150 U/mL. All lipases were
prepared at 150 U/mL in 30% NaBr buffer, and a 0.5 h lipolysis was
performed at the optimal pH and temperature proposed by the manufacturer
in triplicate. As shown in Table 1,
3-MCPD esters could not be
enzymatically decomposed by lipase from Thermophila sparsiformis. Among
the lipases tested, lipase from Candida sp. gave the highest recovery
rate for 3-MCPD. These results suggested that lipase from Candida sp.
had higher substrate specificity for hydrolysis of 3-MCPD esters than
other lipases tested.