Experimental Approach
Wild-type (WT) and NLRP3 knockout (NLRP3-/-) mice were applied to evaluate the protective effects of TBⅡ in DSS-induced mice colitis. The role of TBⅡ in LPS+ATP-induced cell model was evaluated by inhibiting or overexpressing NLRP3. RNA-seq, ELISA, western blots, immunofluorescence staining and the expression analysis by qPCR were performed to examine the alterations of colonic NLRP3 expression in colon tissues and cells, respectively.