2.10 Immunofluorescence staining
Immunofluorescence analysis was performed on the colon tissue. Colon tissues were fixed with 4% paraformaldehyde, permeated with 0.1% Triton-100 for 15min, blocked with 3% bovine serum albumin, and incubated with primary antibody overnight at 4oC. On the second day, fluorescent secondary antibodies (Affinity, Jiangsu, China) were added and incubated at room temperature for 2 h. The nuclei were stained with DAPI (Solarbio, Beijing, China). Finally, imaging was performed under a fluorescence microscope.