2.12 Materials
Dextran sodium sulfate (DSS) was bought from Glycarbo Biomedicals
(California, USA), TBⅡ was obtained from Chroma Biotechnology
Co.,Ltd.(Chengdu, China). Antibodies against NF-κB , p- NF-κB , IKBα,
p-IKBα,NLRP3 were purchased from CST (Boston, USA), Caspase-1 and ASC
were purchased from Santa cruze
(California, USA), IL-1β and GAPDH were purchased from Affinity
(Jiangsu, China), and E-cadherin and Occludin were bought from ABclonal
Biotechnology Co.,Ltd.(WuHan,China). LPS, ATP, MCC950, M-CSF were
purchased from Sigma-Aldrich (St.
Louis, MO, USA). BAY11-7028 was purchased from Med Chem Express
(Monmouth Junction, NJ).