2.2 SOD, GSH and MDA measurement
Colon tissue was cleaned with PBS, homogenized with tissue homogenate
and centrifuged. The activities of SOD, MDA and GSH were determined by
using SOD, GSH and MDA assay kit (Nanjing Jiancheng Bioengineering
Institute, Nanjing, China) according to the manufacturer’s instructions.
2.3 Myeloperoxidase
(MPO) assay
The colon tissue was homogenized and the protein concentration was
quantified with the BCA protein detection kit (Meilunbio, Liaoning,
China). Plasma and colon tissues were measured
according to the instructions for
the myeloperoxidase (MPO) test kit
(Nanjing Jiancheng Bioengineering
Institute, Jiangsu, China). The changes of absorbance was measured at
460nm. In the reaction system at 37oC,
H2O2 was decompose .into 1μmol as one
unit of MPO activity.