2.10 Immunofluorescence staining
Immunofluorescence analysis was performed on the colon tissue. Colon
tissues were fixed with 4% paraformaldehyde, permeated with 0.1%
Triton-100 for 15min, blocked with 3% bovine serum albumin, and
incubated with primary antibody overnight at 4oC. On
the second day, fluorescent secondary antibodies (Affinity,
Jiangsu, China) were added and
incubated at room temperature for 2 h. The nuclei were stained with DAPI
(Solarbio, Beijing, China). Finally, imaging was performed under a
fluorescence microscope.