2.7 Histological analysis and scores
Quickly separate the colon tissue, take pictures, and measure the length of the colon. A small segment of colon was fixed with 4% paraformaldehyde, embedded in paraffin, and sliced 4 μm. After hydration, the sections were stained with hematoxylin and eosin (H&E), and then observed under microscope. The histological score was evaluated according to the scoring criteria.