2.5 Cell cultures and treatment
Primary bone marrow derived macrophages (BMDMs) were obtained from the
femur and tibia bones of C57BL/6J mice (6-8 week), and cultured for 7
days in DMEM (Servicebio, Hubei,
China) containing 10% FBS
(Gbico, New York, USA), 1%
penicillin-streptomycin solution (Gbico, New York, USA) and 10
ng·ml-1 of mouse CSF (Sigma-Aldrich, USA).
HCoEpiC cells were grown in DMEM
containing 10% FBS (Gbico, New York, USA), 1% penicillin-streptomycin
solution (Servicebio, Hubei, China). Both types of two cells were
treated with different concentrations of TBⅡ (25 μM, 50 μM and 100 μM),
BAYBAY11-7082 (20 μM) and MCC950 (10 μM), with or without LPS (500
ng·μL-1) and ATP (0.5 mM).