2.5 Cell cultures and treatment
Primary bone marrow derived macrophages (BMDMs) were obtained from the femur and tibia bones of C57BL/6J mice (6-8 week), and cultured for 7 days in DMEM (Servicebio, Hubei, China) containing 10% FBS (Gbico, New York, USA), 1% penicillin-streptomycin solution (Gbico, New York, USA) and 10 ng·ml-1 of mouse CSF (Sigma-Aldrich, USA). HCoEpiC cells were grown in DMEM containing 10% FBS (Gbico, New York, USA), 1% penicillin-streptomycin solution (Servicebio, Hubei, China). Both types of two cells were treated with different concentrations of TBⅡ (25 μM, 50 μM and 100 μM), BAYBAY11-7082 (20 μM) and MCC950 (10 μM), with or without LPS (500 ng·μL-1) and ATP (0.5 mM).