3.2.1 Triple-label immunocytochemistry
To confirm the presence of three proteins, TRPV1, EP4and B2, in same cell, triple-label immunostaining with
EP4 (blue, 1:200), TRPV1 (green, 1:1000) and
B2 (red, 1:200) were conducted. Confocal images shown in
figure 6A illustrate immunolabelling for the three proteins of interest.
Morphometric analyses of six ganglia have shown that all the three
proteins were present 21.7% of DRG neurons. The size-frequency
histogram presented in figure 6B shows that triple-labelling was limited
to small neurons (96.4%, <1,000 μm2), with
only a small number of medium sized neurones labelled (3.6%, 1,000
-2,000μm2), and no large neurons (>2,000
μm2) labelled. The mean area of labelled neurons was
651.9 ± 15.2 μm2.