3.2.3 Co-localization of B2 and COX-1 in dorsal
root ganglia (DRG)
When the DRG cells were stained with antibodies for both
B2 and COX-1, there was a clear co-expression of the two
proteins. An example of B2 and COX-1 dual labelling is
shown in figure 8A where confocal images have been taken using a
B2 spedific antibody (red) and a COX-1 specific antibody
(green). The histogram of cell size frequency derived for six ganglia
shown in figure 8B shows that 39.2% of DRG neurons express both
B2 receptor and COX-1 proteins, with a mean cell area of
955.7 ± 28.1 μm2.
The dual labelling occurred mainly in small neurons (69.9%,
<1,000 μm2), some medium sized neurons
(23.1%, 1,000-2,000 μm2) and only very few large
neurons (7%, >2,000 μm2) dual labelled.