3.1.1 Enhancing effect of BK and PGE2 on capsaicin evoked responses in isolated DRG neurons
Desensitization was observed upon repeated capsaicin application with a response ratio of 46.9 ± 3.6% (Fig 2A). (PGE (500nM, 3 min) perfused into the bath before the second capsaicin application enhance the capsaicin evoked responses with a response ratio = of 108.7 ± 12.5% (Fig 2B and D). In addition, bradykinin (100nM) via the bath perfusion system has ability to reverse desensitization of capsaicin responses (Fig 2C) with a response ratio of 115±11.8% (Fig 2E). In bradykinin experiments, coverslips were incubated with thapsigargin (2μM) before applying bradykinin in order to block the release of calcium from intracellular stores thus enabling the investigation of the signaling modulation by alternate pathways.