2.1.3 Data analysis:
Treatment effects were statistically analyzed by one-way analysis of variance ANOVA, followed by post-hoc analysis using the Bonferroni correction for multiple comparisons in Graphpad Prism Software (GraphPad Software, La Jolla,California USA). Student’s t-test was used when comparisons were restricted to two experimental groups. A P value of < 0.05 were considered statistically significant.