2.2.3 Co-localization of B2 and COX-1
Dual-label immunocytochemistry was conducted according to the same
procedure as mentioned previously in Section 2.4 The DRG sections were
incubated with the primary antibody for B2 (1:200; Santa
Cruz Biotechnology, Santa Cruz, CA raised in goat) and the COX-1
antibody (1:200; Cayman Chemical) overnight at 4oC.
The DRG sections were then washed with PBS six times for 10 min each.
This was followed by incubation with a host-directed AlexaFluor
conjugated anti-mouse secondary antibody (1:200 dilutions, Jackson
ImmunoResearch Labs) and a Texas Red conjugated anti-goat secondary
antibody (1:200 dilution, Jackson ImmunoResearch Labs). The DRG sections
were then washed again twice with PBS, mounted and photographed.