2.3 Chemicals:
All chemical reagents and solvents were obtained from Sigma, unless otherwise stated. The EP4 and COX-1 antibodies were purchased from Cayman. The bradykinin antibody and RHC-80267 was from Santa Cruz Biotechnology, Inc. H89 and GF109203X were from Enzo Life Science (UK) LTD. SC560 was from Cambridge Biosciences LTD. Capsaicin, bradykinin, GW627368 and L-798106 were purchased from Tocris Bioscience (UK).