2.2.4 Morphometric data analysis
Six sections from six different DRGs from at least different 3 rats were used for morphometric analysis of each experiment. By using a computerized image analysis system (image J), the cell area and the number of labelled and unlabeled cells was determined. The size (in μm2) of DRG neurons was classified based on their cross-sectional area being small (< 1000μm²) or intermediate to large (> 1000μm²) (Chopra et al. 2000, Hammond 2006) using images taken with a Nikon (Labophot 2A) microscope (x 20 objective). A chi-square test was performed to compare the distribution of different proteins. For EP4, COX-1, and B2 localization experiments, data were pooled forms multiple experiments.