2.3 Chemicals:
All chemical reagents and solvents were obtained from Sigma, unless
otherwise stated. The EP4 and COX-1 antibodies were
purchased from Cayman. The bradykinin antibody and RHC-80267 was from
Santa Cruz Biotechnology, Inc. H89 and GF109203X were from Enzo Life
Science (UK) LTD. SC560 was from Cambridge Biosciences LTD. Capsaicin,
bradykinin, GW627368 and L-798106 were purchased from Tocris Bioscience
(UK).