Tests Description Sample used
PCR It is based on NAAT; the gold standard for detecting monkeypox DNA is presently real-time PCR. Lesion fluid
Viral culture From a patient sample, the virus is cultured and isolated. Lesion fluid
Electron microscopy Using an electron microscope, pox viruses are morphologically identified. Biopsy specimen, scab material, vesicular fluid
Immunohistochemistry Antigens specific to the Orthopoxvirus are evaluated for their presence. Biopsy specimen
Anti-Orthopoxvirus IgG and IgM tests These tests may be used to determine if you have been exposed to Orthopoxvirus recently or in the past. Blood specimen