4.5 Limitations and Future Research Directions
Despite the valuable insights generated from this study, certain
limitations and future research directions should be acknowledged:
- Sample size and representation: The limited number of samples (35) and
populations (six distinct cohort groups) included in this study may
not entirely capture the full extent of genetic diversity and
population structure of C. hainanense. Future studies should aim to
increase sample sizes and cover a broader range of fragmented habitats
to provide a more comprehensive understanding.
- Gene flow and landscape connectivity: This study did not investigate
the gene flow and landscape connectivity among the fragmented
habitats. Understanding how landscape features influence gene flow
between populations can provide crucial information for developing
conservation corridors and habitat restoration strategies. Future
research should incorporate landscape genetic approaches to
investigate the impact of habitat fragmentation on gene flow in C.
hainanense populations.
- Functional genetic diversity: The assessment of genetic diversity in
this study primarily focused on neutral genetic markers (SNPs).
However, functional genetic diversity, which reflects the genetic
variation underlying ecologically important traits, is also crucial
for species survival and adaptation. Future studies should explore
functional genetic diversity by incorporating candidate genes or
whole-genome sequencing approaches.
- Long-term monitoring and adaptive management: The dynamics of genetic
diversity and population structure can change over time due to various
factors, such as climate change, anthropogenic disturbances, and
random genetic drift. Long-term monitoring of C. hainanense
populations is essential for detecting changes in genetic diversity
and adjusting conservation strategies accordingly.