4.5 Limitations and Future Research Directions
Despite the valuable insights generated from this study, certain limitations and future research directions should be acknowledged:
  1. Sample size and representation: The limited number of samples (35) and populations (six distinct cohort groups) included in this study may not entirely capture the full extent of genetic diversity and population structure of C. hainanense. Future studies should aim to increase sample sizes and cover a broader range of fragmented habitats to provide a more comprehensive understanding.
  2. Gene flow and landscape connectivity: This study did not investigate the gene flow and landscape connectivity among the fragmented habitats. Understanding how landscape features influence gene flow between populations can provide crucial information for developing conservation corridors and habitat restoration strategies. Future research should incorporate landscape genetic approaches to investigate the impact of habitat fragmentation on gene flow in C. hainanense populations.
  3. Functional genetic diversity: The assessment of genetic diversity in this study primarily focused on neutral genetic markers (SNPs). However, functional genetic diversity, which reflects the genetic variation underlying ecologically important traits, is also crucial for species survival and adaptation. Future studies should explore functional genetic diversity by incorporating candidate genes or whole-genome sequencing approaches.
  4. Long-term monitoring and adaptive management: The dynamics of genetic diversity and population structure can change over time due to various factors, such as climate change, anthropogenic disturbances, and random genetic drift. Long-term monitoring of C. hainanense populations is essential for detecting changes in genetic diversity and adjusting conservation strategies accordingly.