Animals
This experiment utilized adult C57BL/6 mice (Charles-River
Saint-Constant, PQ, Canada) single housed weighing 25 – 35g.
Experiments were conducted between 8:00 and 20:00. All procedures were
in accordance with the ethical guidelines by Memorial University’s
institutional animal care and use committee and the Canadian Institutes
of Health Research. Mice were pseudo-randomized into two factors,
exposure (control or MK-801, n = 30 per factor, 15 males and 15 females)
and treatment (HU-580 - 0.01ug/kg, HU-580 - 0.05ug/kg, or vehicle; n =
20 per level, 10 males and 10 females), creating six groups: Ctrl+Veh,
Ctrl+LowHU, Ctrl+HighHU, MK+Veh, MK+LowHU, and MK+HighHU (n = 10 per
group, 5 males and 5 females).