Effective population size estimation
We used NeEstimator v.2 (Do et al. 2014) via the “dartR” wrapper to
try to estimate the current effective sizes of the King Island and
Tasmanian scrubtit populations, using the linkage disequilibrium method
with no singleton alleles. We also estimated the effective size of (i)
each a-priori scrubtit subpopulation based on sampling locations; and
(ii) the ‘east coast’ and ‘rest of the mainland’ Tasmanian scrubtit
populations. We used this approach based on the results of the
population structure analysis and given the NeEstimator algorithm
assumes random mating and non-overlapping generations (Do et al. 2014)
which are both unlikely to be the case for scrubtits (see results).