Figure 4. (a) Integrated scanning curves of MANP-FLIA with
different PCT concentrations in serum. (b) Calibration curves of
MANP-FLIA with magnetic operation (red), MANP-FLIA without magnetic
operation (black), and AuNPs-LFIA (blue) with different PCT
concentrations in serum. (c) Fluorescent photographs of MANP-FLIA test
strips in response to different concentrations of PCT in serum after
magnetic enrichment and separation (0–250 ng mL−1).
(d) SEM images of the T line region on the NC
membrane after running with
MANP6:6@mAbs at PCT concentrations of
(i) 0 ng mL−1 and
(ii) 100 ng mL−1. (e) Specificity evaluation of the
proposed MANP-FLIA platform toward various inspected species. The
concentration of PCT is 1 ng mL−1, and the
concentration of all the other interfering species is 1000 ng
mL−1. (f) Comparison of the proposed MANP-FLIA method
with the standard clinical CLIA method in actual serum samples. (g)
Correlation analysis between the proposed method and the standard CLIA
method.