Figure 5. (a) Fluorescent photographs, (b) signal responses, and (c) calibration curves of MANP-FLIA test strips in response to different concentrations of LAM (0–20 ng mL−1) in urine with/without magnetic enrichment and separation. (d) Specificity evaluation of the proposed MANP-FLIA platform toward various mycobacteria species with a bacterial concentration of 2.5×104 CFU mL−1. (e) Fluorescent signal of MANP-FLIA when adding different concentration levels of LAM into urine samples provided by 10 individual healthy people. (f) Diagnosis result of clinical TB-positive patients and healthy individuals obtained using MANP-FLIA.