FIGURE
4 Unsupervised clustering of protein patterns distinguishes distinct
grades of gliomas.AMultigroup heatmap with
dendrogram of 4068 LFQ proteins across 13 glioma samples. Ward’s method
was performed for clustering, and Pearson correlation was used for
distance measurement. B Representation of the total intensity
of selected proteins (SYNE1, GLDC, SDPR, CD14, FCGBP, and MEK1) of WHO
grade IV (IV-Mut, n = 4) and II/III (II/III-Mut, n = 3)IDH1 mutant gliomas measured by LC−MS/MS (two-tailed Student’s
t-test, P < 0.05). Error bars indicate standard error
of mean based on biological replicates. C Sankey plot showing
the GSEA results of differentially expressed proteins (DEPs) in GBMs
with IDH1 mutation (IV-Mut, n = 4) and IDH1 wild
type (IV-WT, n = 4). The annotations of GO-BP, Reactome,
WikiPathways, and KEGG pathways are shown (Benjamini−Hochberg FDR
method, adjusted P <
0.01).