3.2 Cell proliferation and immune response are conserved
features for BrMs
The
following four essential hallmarks of metastatic cells during invasion
and brain metastasis have been previously proposed: (a) motility and
invasion; (b) modulation of the microenvironment; (c) plasticity; and
(d) colonization [24].
L.C,
B.C, and C.C are the most common types of cancer that metastasize to the
brain. Here, we performed unsupervised clustering and searched for
robust clustering of distinct features of the proteome correlated with
all BrMs to increase our understanding of the general mechanisms of
BrMs. Hierarchical clustering identified 3 clusters across all samples,
resulting in 79 proteins in cluster 1, 192 proteins in cluster 2, and
125 proteins in cluster 3 (Figure 3A and Table S2). Functional analysis
of the three clusters revealed marked enrichment in cell proliferation
for colonization and immune response to modulate the microenvironment
(Figure 3B,
Figure
S2A and
B).
Importantly,
the
transcriptomic results of BrMs
validate
these
functions
[16].