FIGURE 4 Unsupervised clustering of protein patterns distinguishes distinct grades of gliomas.AMultigroup heatmap with dendrogram of 4068 LFQ proteins across 13 glioma samples. Ward’s method was performed for clustering, and Pearson correlation was used for distance measurement. B Representation of the total intensity of selected proteins (SYNE1, GLDC, SDPR, CD14, FCGBP, and MEK1) of WHO grade IV (IV-Mut, n = 4) and II/III (II/III-Mut, n = 3)IDH1 mutant gliomas measured by LC−MS/MS (two-tailed Student’s t-test, P < 0.05). Error bars indicate standard error of mean based on biological replicates. C Sankey plot showing the GSEA results of differentially expressed proteins (DEPs) in GBMs with IDH1 mutation (IV-Mut, n = 4) and IDH1 wild type (IV-WT, n = 4). The annotations of GO-BP, Reactome, WikiPathways, and KEGG pathways are shown (Benjamini−Hochberg FDR method, adjusted P < 0.01).