Flow cytometry
SH-SY5Y apoptosis when co-cultured with BV2 cells was determined by flow
cytometry. Specifically, SH-SY5Y cells were stained with Annexin and
propidium iodide (PI; Multi Sciences, AP101, China) according to the
manufacturer’s manual. Stained cell preparations were run and analyzed
on a flow cytometer (CytoFLEX, Beckman, USA).