Behavioral tests
The rotarod test was applied to examine animal strength and coordination. Each mouse was placed on an accelerated rotating rod (30 revolutions per minute) and the latency period to fall from the rod was recorded. If the subject did not fall from the rod within 300 seconds, a maximum of 300 seconds was recorded. Each experiment was repeated three times and the mean value was calculated.
A ball wrapped with gauze was fixed on a rough rod (diameter: 0.8cm; Height: 60 cm). Mice were placed on the ball with its head vertically up. The time it took to turn its head completely downward was recorded as T-turn, and the time it took to climb down to the ground was recorded as T-D. If the mouse fell off the pole, the data was not recorded. When mice stayed on the pole for more than 120 seconds, a maximum value of 120 seconds was recorded.