Flow cytometry
SH-SY5Y apoptosis when co-cultured with BV2 cells was determined by flow cytometry. Specifically, SH-SY5Y cells were stained with Annexin and propidium iodide (PI; Multi Sciences, AP101, China) according to the manufacturer’s manual. Stained cell preparations were run and analyzed on a flow cytometer (CytoFLEX, Beckman, USA).