Enzyme-linked immunosorbent assay (ELISA) analysis
Part of the frozen colon tissue was obtained and placed in the lysate
with a tissue crusher to break the homogenate. The lysates were
centrifuged at 8000g for 10 min at 4 ℃, and the supernatants were
transferred for ELISA. Human TNF-α, Mouse TNF-α, IL-1β, MCP-1 and IL-6
concentrations were determined using the ELISA MAX™ Deluxe set
(BioLegend, USA) according to the manufacturer’s instructions.