Figure 5 The Notch signaling pathway was weakened by treatment
with EGCG in colon of the UC mice. The tissue obtained from the colon
of mice was homogenized in a protein extraction solution; 60 μg of each
sample was used for detection. (A ) The expression levels of
Notch1, Notch2, Cleaved-Notch1, and Hes-1 were measured by western
blotting, and the ODs (B, C, D, E) were detected. (F ) IHC was
performed on paraffin-embedded sections stained for Notch1 and Hes-1:
brown area (red arrows) indicates positive staining. Data are pooled
from two experiments (n=6 colon from six mice per group, repeated
twice). Data are presented as the means ± SEM. *P<0.05,
**P<0.01, ***P<0.001 (one-way ANOVA, EGCG, and
normal group vs. DSS group). Scale bars, 100 μm. LE, Low dose of
(-)-epigallocatechin gallate; HE, High dose of (-)-epigallocatechin
gallate; DSS, Dextran sulfate sodium.