Enzyme-linked immunosorbent assay (ELISA) analysis
Part of the frozen colon tissue was obtained and placed in the lysate with a tissue crusher to break the homogenate. The lysates were centrifuged at 8000g for 10 min at 4 ℃, and the supernatants were transferred for ELISA. Human TNF-α, Mouse TNF-α, IL-1β, MCP-1 and IL-6 concentrations were determined using the ELISA MAX™ Deluxe set (BioLegend, USA) according to the manufacturer’s instructions.